What Is L-Asparagine Monohydrate? Applications, Dosage, Alternatives & Specifications

L-Asparagine Monohydrate, chemically known as (S)-2,4-Diamino-4-oxobutanoic acid monohydrate, is the β-hydroxyamide derivative of L-aspartic acid. It is an important category of proteinogenic amino acids and one of the 20 standard amino acids, with wide application value in the global pharmaceutical industry, food industry, cosmetic industry, and biotechnology field. Its molecular formula is C4H8N2O3·H2O, molecular weight is 150.13, CAS number is 5794–13–8, and EINECS number is 200–735–9. L-Asparagine is naturally present in various proteins, has a high nitrogen-to-carbon ratio, and is a key regulatory factor for nitrogen storage and transport in living organisms. It serves as a precursor to several essential chemicals in the Krebs cycle and participates in the synthesis and transformation of amino acids in vivo. It often exists as a monohydrate, appearing as white orthorhombic crystals or crystalline powder, odorless, with a special sweet taste. L-Asparagine is a non-essential amino acid in humans and can be synthesized from aspartic acid via amidation with ammonia. However, under certain physiological conditions (such as tumor growth or stress) or in cell culture, exogenous supplementation is crucial for maintaining normal metabolism and function. L-Asparagine is also the core substrate for L-Asparaginase, a key drug for treating acute lymphoblastic leukemia (ALL). Compared to other amino acids, L-asparagine‘s unique feature is its high nitrogen-to-carbon ratio, enabling it to effectively transport and store nitrogen, supporting rapid cell proliferation and protein synthesis. Structurally, L-asparagine has an amide group (-CONH2) in its side chain, making it uncharged at neutral pH. This structural difference gives it distinct physicochemical properties and physiological functions compared to aspartic acid. It has high solubility in water and buffers, making it a very effective protein structure stabilizer. It is widely used in cell culture formulations and has important strategic value in the biomedical field, especially in cell culture and biopharmaceuticals.

L-Asparagine Monohydrate is recognized as the golden amino acid additive for cell culture, holding an increasingly important position in modern biopharmaceuticals, the food industry, and medicine. Its most outstanding core advantages lie in its unique nitrogen metabolism regulation function, excellent cell culture suitability, and extremely wide application adaptability.

Core Nutritional and Physiological Regulation Functions: L-Asparagine has a high nitrogen-to-carbon ratio and is a key regulatory factor for nitrogen storage and transport in living organisms. This property gives it a unique advantage in cellular nitrogen metabolism regulation and amino acid balance, especially in promoting cell growth and protein synthesis.